Journal
BIORESOURCE TECHNOLOGY
Volume 235, Issue -, Pages 316-324Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.03.104
Keywords
Antibiotic resistance; Enterococci; Pharmaceutical waste sludge; Anaerobic digestion; Pretreatment
Funding
- National Natural Science Foundation of China [51578535, 21207147]
- National Major Science & Technology Projects for Water Pollution Control and Management of China [2015ZX07203-005]
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Pharmaceutical waste sludge harbors large amounts of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs), and it is necessary to study the reduction of ARGs and MGEs during sludge treatment. Therefore, the antibiotic resistance phenotypes and genotypes of enterococci, and the ARGs and MGEs in genomic DNA were investigated during anaerobic digestion (AD) with microwave (MW), thermal hydrolysis (TH) and ozone pretreatment. Results showed that sludge pretreatment increased the occurrence of the resistance phenotypes and genotypes of enterococci. During AD, the resistance of enterococci to macrolides decreased, except for in the MW-pretreated sludge. Horizontal gene transfer and co-occurrence of ermB and tetM in enterococci resulted in increased tetracycline resistance of enterococci throughout the sludge treatment. MGEs such as intI1, ISCR1 and Tn916/1545 had a significant effect on the distribution of ARGs. AD with pretreatment, especially TH pretreatment, resulted in greater ARGs and MGEs reduction and improved methane production. (C) 2017 Published by Elsevier Ltd.
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