Journal
BIORESOURCE TECHNOLOGY
Volume 235, Issue -, Pages 12-17Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.03.088
Keywords
Alkali-pretreated SCB; Solvent water; Oleyl alcohol; Fed-batch strategy; Enzymatic saccharification; High solids loading
Funding
- Program of National Natural Science Foundation of China [21306196, 21476233]
- Natural Science Basic Research Plan in Shaanxi Province of China [2015JM3098]
- Guangdong Science and Technology Research Program [2013B010403021]
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Solvent water is an essential factor for high solids enzymatic hydrolysis. To investigate its effect on substrate conversion efficiency in high solids hydrolysis of sugarcane bagasse (SCB), oleyl alcohol was used to partially substitute the solvent water. The results in batch hydrolysis tests in which diverse ratio of solvent water was replaced found that the majority of the substrate was insoluble. Then high solids fedbatch hydrolysis with the reaction solution mixed with solvent water and oleyl alcohol in the ratio of 3: 1 (solids concentration correspond to 24% (w/v)) was carried out at the final real solids loading of 18% (w/v). The produced sugars were found to be less than pure water system, which indicated that water played a significant role in high solids hydrolysis process, and solids effect was related to the solvent water content. (C) 2017 Elsevier Ltd. All rights reserved.
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