Journal
RSC ADVANCES
Volume 7, Issue 61, Pages 38699-38713Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7ra05044e
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Herein, we describe the green synthesis of silver nanoparticles (AgNPs) using Ocimum basilicum L. var. thyrsiflora leaf derived callus extracts formed in response to 9.0 mu M of thidiazuron (TDZ) alone and a combination of 9.0 mu M of TDZ + 15 mu M of melatonin (Mel) which act both as reducing and stabilizing agents. Based on the phytochemical analysis, the highest total phenolic and flavonoid content were recorded (44.9 mg g(-1) DW and 31.5 mg g(-1) DW, respectively) in the callus grown on TDZ (9.0 mu M) + Mel (15 mu M) compared to the TDZ alone (29.8 mg g(-1) DW and 17.6 mg g(-1) DW, respectively) induced callus. The biosynthesized AgNPs were confirmed by analyzing the excitation of surface plasmon resonance using ultraviolet-visible spectroscopy at 420-430 nm. The synthesized AgNPs were characterized via Xray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, and energy dispersive X-ray spectroscopy, which indicate that more potent antibacterial and antileishmanial activity is displayed by the Mel plus TDZ callus extract mediated AgNPs than the TDZ-only callus extract mediated AgNPs against multiple drug resistant human pathogens and Leishmania major. Determination of the antileishmanial efficacy of AgNPs is a major step towards the development of new compounds containing nanoparticles for leishmaniasis treatment.
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