Journal
ANALYTICA CHIMICA ACTA
Volume 972, Issue -, Pages 28-37Publisher
ELSEVIER
DOI: 10.1016/j.aca.2017.04.035
Keywords
Electrochemical nanosensor; CdSe quantum dots; Uric acid; Creatinine; Hollow fiber; Taguchi method
Categories
Funding
- Payame Noor University of Mashhad, Iran
Ask authors/readers for more resources
In this research, a novel sensitive electrochemical nanosensor based on the cadmium selenide quantum dots (QDs)/ionic liquid mediated hollow fiber-pencil graphite electrode (HF-PGE) was prepared and applied for simultaneous determination of uric acid (UA) and creatinine (Crn) in urine and serum samples. The electrocatalytic oxidation of the analytes was investigated via differential pulse (DPV) and cyclic voltammetry (CV). The experiments were designed, in two different steps, according to Taguchi's method; OA9 L9 (33) and OA9 L9 (34) orthogonal array to optimize experimental runs. The results revealed that the electrode response was initially influenced by the types of sensor and types of ionic liquids and their ratios. The amount of QD, buffer pH, equilibration time and scan rate also influenced electrode response efficiency. According to the results of Taguchi analysis, the amount of tetra phenyl phosphonium chloride (TPPC) and QD were the most influencing parameters on the yield response of the modified electrodes. Linear ranges were obtained between 0.297-2.970 x 10(3) and 0.442 -8.840 x 10(3) mu M, with the detection limits of 0.083 and 0.229 mM and relative standard deviations (RSD) of 2.4% and 1.8%, for UA and Crn, respectively. Finally, the proposed method was successfully examined for simultaneous determination of UA and Crn in human urine and serum samples. (C) 2017 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available