Journal
CARBOHYDRATE POLYMERS
Volume 166, Issue -, Pages 173-182Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2017.02.105
Keywords
Chitosan; Hydroxyapatite; pH-responsive hydrogel; Rheology; Cell encapsulation
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Funding
- Croatian Science Foundation [IP-2014-09-3752]
- Spanish Ministry of Economy and Competitiveness (Feder funds) [MAT2016-76039-C4-1-R]
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The development of bioactive injectable system as cell carrier with minimal impact on viability of encapsulated cells represents a great challenge. In the present work, we propose a new pH-responsive chitosan-hydroxyapatite-based hydrogel with sodium bicarbonate (NaHCO3) as the gelling agent. The in situ synthesis of hydroxyapatite phase has resulted in stable composite suspension and final homogeneous hydrogel. The application of sodium bicarbonate has allowed non-cytotoxic fast gelation of chitosan-hydroxyapatite within 4min, and without excess of sodium ions concentration. Rheological properties of crosslinked hydrogel have demonstrated possible behaviour as 'strong physical hydro gel'. The live dead staining has confirmed good viability and dispersion, as well as proliferation of encapsulated cells by the culture time. Presented preliminary results show good potential of chitosanhydroxyapatite/NaHCO3 as a cell carrier, whose impact on the cell differentiation need to be confirmed by encapsulation of other cell phenotypes. (C) 2017 Elsevier Ltd. All rights reserved.
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