Journal
DEVELOPMENTAL BIOLOGY
Volume 426, Issue 2, Pages 219-235Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2016.03.005
Keywords
-
Categories
Funding
- National Eye Institute of the National Institutes of Health [R01EY017964, R01EY015748]
- Hendricks Bridge Grant Award
- Ministry of Economy and Competitiveness of Spain [RYC-2011-09321]
- ERDF [CGL2012-40082]
- Research to Prevent Blindness
- Lions Club of Central New York
Ask authors/readers for more resources
Intermediate filament proteins are structural components of the cellular cytoskeleton with cell-type specific expression and function. Glial fibrillary acidic protein (GFAP) is a type III intermediate filament protein and is up-regulated in glia of the nervous system in response to injury and during neurode-generative diseases. In the retina, GFAP levels are dramatically increased in Muller glia and are thought to play a role in the extensive structural changes resulting in Muller cell hypertrophy and glial scar formation. In spite of similar changes to the morphology of Xenopus Muller cells following injury, we found that Xenopus lack a gfap gene. Other type III intermediate filament proteins were, however, significantly induced following rod photoreceptor ablation and retinal ganglion cell axotomy. The recently available X. tropicalis and X. laevis genomes indicate a small deletion most likely resulted in the loss of the gfap gene during anuran evolution. Lastly, a survey of representative species from all three extant amphibian orders including the Anura (frogs, toads), Caudata (salamanders, newts), and Gymnophiona (caecilians) suggests that deletion of the gfap locus occurred in the ancestor of all Anura after its divergence from the Caudata ancestor around 290 million years ago. Our results demonstrate that extensive changes in Muller cell morphology following retinal injury do not require GFAP in Xenopus, and other type III intermediate filament proteins may be involved in the gliotic response. (C) 2016 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available