Journal
BIOSENSORS & BIOELECTRONICS
Volume 92, Issue -, Pages 26-32Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.01.057
Keywords
Fluorescence resonance energy transfer; Multiwalled carbon nanotubes@graphene oxide nanoribbons; Quantum dots; On-Off-On switching; Transgenic soybean
Categories
Funding
- National Natural Science Foundation of China [21375050, 21405063, 21505055, 21675066]
- Jiangsu University [5503000011]
- Natural Science Foundation of the Jiangsu Higher Education Institutions, China [15KJA550001]
- Innovation Project of Science and Technology for College Graduates of Jiangsu Province [KYLX15_1091]
- China Postdoctoral Science Foundation [2015M581745, 2015M580401]
- Jiangsu Province Postdoctoral Science Foundation [1501108C]
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With the increasing concern of potential health and environmental risk, it is essential to develop reliable methods for transgenic soybean detection. Herein, a simple, sensitive and selective assay was constructed based on homogeneous fluorescence resonance energy transfer (FRET) between CdTe quantum dots (QDs) and multiwalled carbon nanotubes@graphene codde nanoribbons.(MWCNTs@GONRs) to form the fluorescent on-off-on switching for simultaneous monitoring dual target DNAs of promoter cauliflower mosaic virus 35 s (P35s) and terminator nopaline synthase (TNOS) from transgenic soybean. The capture DNAs were immobilized with corresponding QDs to obtain strong fluorescent signals (turning on). The strong pi-pi stacking interaction between single-stranded DNA (ssDNA) probes and MWCNTs@GONRs led to minimal background fluorescence due to the FRET process (turning off). The targets of P35s and TNOS were recognized by dual fluorescent probes to form double-stranded DNA (dsDNA) through the specific hybridization between target DNAs and ssDNA probes. And the dsDNA were released from the surface of MWCNTs@GONRs, which leaded the dual fluorescent probes to generate the strong fluorescent emissions (turning on). Therefore, this proposed homogeneous assay can be achieved to detect P35s and TNOS simultaneously by monitoring the relevant fluorescent emissions. Moreover, this assay can distinguish complementary and mismatched nucleic acid sequences with high sensitivity. The constructed approach has the potential to be a tool for daily detection of genetically modified organism with the merits of feasibility and reliability.
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