Journal
BIOSENSORS & BIOELECTRONICS
Volume 92, Issue -, Pages 335-341Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.11.003
Keywords
In situ; FRET; Upconversion nanoparticles; Whole blood; Immunoassay
Categories
Funding
- NSF of China [11174277, 11374297, 61275202, 21304084, 11474278, 61575194, 11504371, 11604331, 11674316, 51372096]
- CAS of China
- KNAW of the Netherlands
- IOP program of the Netherlands
- John van Geuns foundation
- NWO TA program of the Netherlands
- Europe MSCA-ITN-ETN Action program, ISPIC [675742]
- Netherlands Organisation for Scientific Research [731.015.206]
- European Union COST Action [CM1403]
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Despite their general clinical applications, current fluorescence-based immunoassays are confronted with serious challenges, e.g. the advance serum/ plasma separation and the tedious washing process in current heterogeneous approaches, and aggregation of particles, low sensitivity and the narrow linear range in homogeneous approaches. In this paper, these urgent problems were solved in a novel one-step in situ immunoassay of whole blood samples by combining the traditional fluorescence resonance energy transfer (FRET) technology (between upconversion nanoparticles (UCNPs) and gold nanoparticles (GNPs)) and the solid-substrate based immunoassay technology. The low detection limits of goat IgG (gIgG) as 0.042 mu g/mL in buffers, 0.51 mu g/mL in 20-fold diluted whole blood samples and a wide linear range from 0.75 mu g/mL to 60 mu g/mL in blood samples were achieved. To the best of our knowledge, it is the first one-step in situ solid-substrate based immunoassay of whole blood samples with large linear detection range. This development provides a promising platform for a rapid and sensitive immunoassay of various bio-molecules directly in whole blood without tedious separation, washing steps and aggregation problems.
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