Journal
BIOSENSORS & BIOELECTRONICS
Volume 92, Issue -, Pages 434-441Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.10.083
Keywords
3-aminophenyl boronic acid; PPy-Ag@BSA; Impedimetric cytosensor; Sialic acid; Renal cell carcinoma
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Funding
- National Natural Science Foundation of China [81370403, 31571554]
- Chongqing Foundation and Advanced Research [CSTC2015jcyjBX0053]
- Chongqing Medical University Scientific Research Cultivating Fund [201414]
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Renal cell carcinoma (RCC) often expresses a high density of sialic acid-rich glycoproteins which helps these late-stage cancer cells to enter the blood stream or urine. Blood diagnosis is a complex and time-consuming process. In this study, we developed a facile and non-invasive electrochemical cytosensor for early detection of RCC in urine samples based on specific recognition by 3-aminophenyl boronic acid (APBA). Polypyrrole (PPy) and bovine serum albumin (BSA)-incorporated Ag submicron particles (Ag@BSA) were co-deposited on a gold electrode (GE) to take advantages of the excellent properties of these biomaterials, including good biocompatibility, low cytotoxicity and excellent electro-conductivity. To further increase the biosensor's sensitivity, APBA molecules were integrated to recognize sialic acid (SA) on the cell surface. Under optimal conditions, the impedimetric cytosensor exhibited a good linear relationship with the logarithm of the cell concentration from 17 to 1.7x10(6) cells mL(-1), and the low detection limit was 6 cells mL(-1) (S/N=3). Therefore, the electrochemical impedimetric biosensor offers a potential approach to bedside rapid detection of RCC in clinical applications.
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