Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 9, Issue 19, Pages 15995-16005Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b03371
Keywords
MoS2 nanoflakes; dendrimers; photothermal therapy; gene silencing; tumors
Funding
- National Natural Science Foundation of China [21273032, 81571679, 81271596]
- Fundamental Research Funds for the Central Universities
- Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning
- FCT-Foundation for Science and Technology [PEst-OE/QUI/U10674/2013]
- Madeira Chemistry Center - CQM + (Madeira) [M1420-01-0145-FEDER-000005]
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Exploitation of novel hybrid nanomaterials for combinational tumor therapy is challenging. In this work, we synthesized dendrimer-modified MoS2 nanoflakes for combinational gene silencing and photothermal therapy (PTT) of cancer cells. Hydro thermally synthesized MoS2 nanoflakes were modified with generation 5 (G5) poly(amidoamine) dendrimers partially functionalized with lipoic acid via disulfide bond. The formed G5-MoS2 nanoflakes display good colloidal stability and superior photothermal conversion efficiency and photothermal stability. With the dendrimer surface amines on their surface, the GS-MoS2 nanoflakes are capable of delivering Bcl-2 (B-cell lymphoma-2) siRNA to cancer cells (4T1 cells, a mouse breast cancer cells) with excellent transfection efficiency, inducing 47.3% of Bcl-2 protein expression inhibition. In vitro cell viability assay data show that cells treated with the G5-MoS2/Bcl-2 siRNA polyplexes under laser irradiation have a viability of 21.0%, which is much lower than other groups of single mode PTT treatment (45.8%) or single mode of gene therapy (68.7%). Moreover, the super efficacy of combinational therapy was further demonstrated by treating a xenografted 4T1 tumor model in vivo. These results suggest that the synthesized G5-MoS2 nanoflakes may be employed as a potential nanoplatform for combinational gene silencing and PTT of tumors.
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