Journal
BIOSENSORS & BIOELECTRONICS
Volume 94, Issue -, Pages 141-147Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.03.001
Keywords
Tumor marker; Dual-signal channel; Electrochemical immunosensing; Au-CoS; Mesoporous CeO2; PSA
Categories
Funding
- National Natural Science Foundation of China [21375047, 21377046, 21575050, 21505051]
- Science and Technology Plan Project of Jinan [201307010]
- Science and Technology Development Plan of Shandong Province [2014GSF120004]
- Special Project for Independent Innovation and Achievements Transformation of Shandong Province [2014ZZCX05101]
- Doctoral and Postdoctoral Science Foundation of University of Jinan
- Special Foundation for Taishan Scholar Professorship of Shandong Province [ts20130937]
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Combining dual-signal channels of differential pulse voltammetry (DPV) and amperometric i-t curve, we developed an amplified sandwich-type electrochemical immunosensor for ultrasensitive detection of prostate specific antigen (PSA). Due to the large specific surface area and good adsorption property, 3-aminopropyl-triethox functionalized CeO2 mesoporous nanoparticles (NH2-M-CeO2) was used for supporting toluidine blue (TB) as the electron transfer mediator and anti-PSA as the signal response. To prevent the leak of TB and facilitate the electron transfer property of NH2-M-CeO2, ionic liquids doped carboxymethyl chitosan (CMC/ILs) were incorporated and TB/M-CeO2/CMC/ILs complex was gotten to act as the label of anti-PSA (Ab(2)). Meanwhile, Au-CoS/graphene was as the matrix materials modifying the electrode to immobilize anti-PSA (Ab(1)). Specifically, Au-CoS/graphene cannot produce electrochemical signals through DPV method but can provide an obviously electrochemical signal towards catalysis hydrogen peroxide (H2O2) through amperometric i-t curve method. At the time of detection, with the increase of concentrations of PSA, the DPV signals from TB/M-CeO2/CMC/ILs were increased and the amperometric i-t curve signals from the Au-CoS/graphene were decreased. Under the optimum conditions, the immunosensor demonstrated remarkable analytical performance of a linear range of 0.5 pg/mL to 50 ng/mL with a detection limit of 0.16 pg/mL for quantitative detection of PSA (S/N=3).
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