Journal
BIOSENSORS & BIOELECTRONICS
Volume 94, Issue -, Pages 365-373Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.02.038
Keywords
Genosensor; Graphene/Zinc Oxide Nanocomposite; Methylene Blue; RNA Detection; Impedance
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Funding
- Ministry of Higher Education (FRGS grant) [FRGS/2/2014/SG06/UNIM/03/1]
- HIR-Chancellory UM [UM.C/625/1/HIR/079]
- HIR-MOHE [UM.C/625/1/HIR/MOHE/SC/06]
- University of Nottingham
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An efficient electrochemical impedance genosensing platform has been constructed based on graphene/zinc oxide nanocomposite produced via a facile and green approach. Highly pristine graphene was synthesised from graphite through liquid phase sonication and then mixed with zinc acetate hexahydrate for the synthesis of graphene/zinc oxide nanocomposite by solvothermal growth. The as-synthesised graphene/zinc oxide nano composite was characterised with scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman spectroscopy and X-ray diffractometry (XRD) to evaluate its morphology, crystallinity, composition and purity. An amino-modified single stranded DNA oligonucleotide probe synthesised based on complementary Coconut Cadang-Cadang Viroid (CCCVd) RNA sequence, was covalently bonded onto the surface of graphene/zinc oxide nanocomposite by the bio-linker 1-pyrenebutyric acid N-hydroxysuccinimide ester. The hybridisation events were monitored by electrochemical impedance spectroscopy (EIS). Under optimised sensing conditions, the single stranded CCCVd RNA oligonucleotide target could be quantified in a wide range of 1.0x 10(-11) M to 1.0%10(-6) with good linearity (R =0.9927), high sensitivity with low detection limit of 4.3x10(-12) M. Differential pulse voltammetry (DPV) was also performed for the estimation of nucleic acid density on the graphene/zinc oxide nanocomposite-modified sensing platform. The current work demonstrates an important advancement towards the development of a sensitive detection assay for various diseases involving RNA agents such as CCCVd in the future.
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