Journal
BIOSENSORS & BIOELECTRONICS
Volume 94, Issue -, Pages 507-512Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2017.03.048
Keywords
DNA biosensor; Carbon dots; Banana peels; Pd-Au@Carbon dots nanocomposite
Categories
Funding
- National Natural Science Foundation of China [51673113, 51273108]
- National Basic Research Program of China (973 Program) [2013CB834803]
- Education Bureau of Fujian Province [JAT160875]
- Natural Science Foundation of Zhangzhou [ZZ2016J31]
- Training Program Foundation for Excellent Youth Researching Talents of Fujian's Universities (Fujian Education Section) [201623]
- open fund of Fujian Provincial Key Laboratory of Modern Analytical Science and Separation Technology (Minnan Normal University)
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In this study, a green and fast method was developed to synthesize high-yield carbon dots (CDs) via one-pot microwave treatment of banana peels without using any other surface passivation agents. Then the as-prepared CDs was used as the reducing agent and stabilizer to synthesize a Pd-Au@CDs nanocomposite by a simple sequential reduction strategy. Finally, Pd-Au@CDs nanocomposite modified glassy carbon electrode (Pd-Au@ CDs/GCE) was obtained as a biosensor for target DNA after being immobilized a single-stranded probe DNA by a carboxyl ammonia condensation reaction. Under the optimal conditions, the sensor could detect target DNA concentrations in the range from 5.0x10(-16) to 1.0x10(-1)degrees mol L-1. The detection limit (LD) was estimated to be 1.82x10(-17) mol L-1, which showed higher sensitivity than other electrochemical biosensors reported. In addition, the DNA sensor was also successfully applied to detect colitoxin DNA in human serum.
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