Journal
EMBO JOURNAL
Volume 36, Issue 11, Pages 1559-1576Publisher
WILEY
DOI: 10.15252/embj.201696392
Keywords
bZIP; diurnal; nitrogen; Phaeodactylum tricornutum; tricarboxylic acid cycle
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Funding
- Agency for Innovation by Science and Technology in Flanders (Strategisch Basisonderzoek) [80031]
- European Union COST Action [FA1006-PlantEngine]
- Max Planck Society
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Diatoms are amongst the most important marine microalgae in terms of biomass, but little is known concerning the molecular mechanisms that regulate their versatile metabolism. Here, the pennate diatom Phaeodactylum tricornutum was studied at the metabolite and transcriptome level during nitrogen starvation and following imposition of three other stresses that impede growth. The coordinated upregulation of the tricarboxylic acid (TCA) cycle during the nitrogen stress response was the most striking observation. Through co-expression analysis and DNA binding assays, the transcription factor bZIP14 was identified as a regulator of the TCA cycle, also beyond the nitrogen starvation response, namely in diurnal regulation. Accordingly, metabolic and transcriptional shifts were observed upon overexpression of bZIP14 in transformed P. tricornutum cells. Our data indicate that the TCA cycle is a tightly regulated and important hub for carbon reallocation in the diatom cell during nutrient starvation and that bZIP14 is a conserved regulator of this cycle.
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