In Silico analysis of piperine, resveratrol, and vanillic acid on NF-κB p65 protein expression

The NF-kappa B is family of transcription factor has important role in innate immunity and inflamation which regulate expression of both pro-and anti-inflammatory mediators. Dysregulation of NF-kappa B pathways results in severe diseases such as arthritis, immunodeficiency, autoimmunity, and cancer. Hence, a method of inhibiting NF-kappa B signaling has potential therapeutic application in cancer and inflammatory diseases. Naturally, obtain bioactive compounds such as piperine, vanillic acid and resveratrol have ample of scope in this area due to their safety and efficacy. In the present study, selected bioactive compounds investigated for their binding affinity with NF kappa B p65 using AutoDock 4.2 software. Our obtained results revealed that piperine has better binding energy -7.41 Kcal/mol and IC50 activity 3.67 mu mol/KI towards NF-kappa B p65 as compared to resveratrol and vanillic acid. These results could help to understand the type of interaction between bioactive compounds with NF-kappa B p65 binding sites.