4.8 Article

Toward a Droplet-Based Single-Cell Radiometric Assay

Journal

ANALYTICAL CHEMISTRY
Volume 89, Issue 12, Pages 6472-6481

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b00414

Keywords

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Funding

  1. National Institutes of Health [NIH NCI 1R21CA193001]
  2. Stanford ChEM-H seed grant
  3. Damon Runyon Cancer Research Foundation [DRR-36-15]
  4. NCI Training Grant [T32 CA118681]

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Radiotracers are widely used to track molecular processes, both in vitro and in vivo, with high sensitivity and specificity. However, most radionuclide detection methods have spatial resolution inadequate for single-cell analysis. A few existing methods can extract single-cell information from radioactive decays, but the stochastic nature of the process precludes high-throughput measurement (and sorting) of single cells. In this work, we introduce a new concept for translating radioactive decays occurring stochastically within radiolabeled single-cells into an integrated, long-lasting fluorescence signal. Single cells are encapsulated in radiofluorogenic droplets containing molecular probes sensitive to byproducts of ionizing radiation (primarily reactive oxygen species, or ROS). Different probes were examined in bulk solutions, and dihydrorhodamine 123 (DHRh 123) was selected as the lead candidate due to its sensitivity and reproducibility. Fluorescence intensity of DHRh 123 in bulk increased at a rate of 54% per Gy of X-ray radiation and IS% per MBq/ml of 2-deoxy-2-[F-18]fluoro-D-glucose ([F-18]FDG). Fluorescence imaging of microfluidic droplets showed the same linear response, but droplets were less sensitive overall than the bulk ROS sensor (detection limit of 3 Gy per droplet). Finally, droplets encapsulating radiolabeled cancer cells allowed, for the first time, the detection of [F-18]FDG radiotracer uptake in single cells through fluorescence activation. With further improvements, we expect this technology to enable quantitative measurement and selective sorting of single cells based on the uptake of radiolabeled small molecules.

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