Journal
ANALYTICAL CHEMISTRY
Volume 89, Issue 11, Pages 6147-6153Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.7b00943
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Funding
- Ministry of Science and Technology (MOST), R.O.C. [MOST 105-2113-M-002-004-MY2]
- Taipei Veterans General Hospital, Taipei, Taiwan [V106C-130]
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Blood testing for endogenous small metabolites to determine physiological and biochemical states is routine for laboratory analysis. Here we demonstrate that by combining the commercial direct analysis in real time (DART) ion source with an ion trap mass spectrometer, native cholesterol in its free alcohol form is readily detected from a few hundred nanoliters of human serum loaded onto chromatography paper. Deuterium labeled cholesterol was used as the internal standard to obtain the absolute quantity of the endogenous cholesterol. The amount of the cholesterol measured by this paper-loaded DART mass spectrometry (pDART-MS) is statistically comparable with that obtained by using commercially available fluorometric-enzymatic assay and liquid chromatography/mass spectrometry. Furthermore, sera from participants at three different time points in an ultramarathon were collected to obtain their cholesterol levels. The test requires only very minimal sample preparation, and the concentrations of cholesterol in each sample were acquired within a minute.
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