Journal
ONCOTARGET
Volume 8, Issue 27, Pages 44096-44107Publisher
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.17307
Keywords
breast cancer; RNA; noncoding RNA; circRNA; biomarker
Categories
Funding
- National Natural Science Foundation of China [81172268]
- Social Development Project in Jiangsu Province [BE2015694]
- Scientific Research Innovation Project for Graduate Students in Jiangsu Province [KYLX16_1125]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAAD)
Ask authors/readers for more resources
Endogenous noncoding circular RNAs (circRNAs) have gained attention for their involvement in carcinogenesis, but their expression pattern in breast cancer has remained largely unknown. In this two-stage study, we first used an Arraystar Human circRNA Array to construct a genome-wide circRNA profile. We then selected candidate circRNAs for validation using a quantitative real-time polymerase chain reaction system. CircRNA/miRNA interactions were predicted and sequence analyses were performed. Among 1155 differentially expressed circRNAs, 715 were upregulated and 440 were downregulated in breast cancer tissues. The validation study demonstrated that hsa_ circ_103110, hsa_circ_104689 and hsa_circ_104821 levels were elevated in breast cancer tissues, whereas hsa_circ_006054, hsa_circ_100219 and hsa_circ_406697 were downregulated. These circRNAs targeted complementary miRNA response elements. The area under the receiver operating characteristic curve for distinguishing breast cancer was 0.82 (95% CI: 0.73-0.90) when hsa_circ_006054, hsa_circ_100219 and hsa_ circ_406697 were used in combination. This study provides evidence that circRNAs are differentially expressed in breast cancer and are important in carcinogenesis because they participate in cancer-related pathways and sequester miRNAs.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available