6-Shogaol attenuates LPS-induced inflammation in BV2 microglia cells by activating PPAR-γ

6-Shogaol, a pungent agent isolated from Zingiber officinale Roscoe, has been known to have anti-tumor and anti-inflammatory effects. However, the anti-inflammatory effects and biological mechanism of 6-Shogaol in LPS-activated BV2 microglia remains largely unknown. In this study, we evaluated the anti-inflammatory effects of 6-Shogaol in LPS-activated BV2 microglia. 6-Shogaol was administrated 1 h before LPS treatment. The production of inflammatory mediators were detected by ELISA. The expression of NF-kappa B and PPAR-gamma were detected by western blot analysis. Our results revealed that 6-Shogaol inhibited LPS-induced TNF-alpha, IL-1 beta, IL-6, and PGE2 production in a concentration dependent manner. Furthermore, 6-Shogaol inhibited LPS-induced NF-kappa B activation by inhibiting phosphorylation and nuclear translocation of NF-kappa B p65. In addition, 6-Shogaol could increase the expression of PPAR-gamma. Moreover, inhibition of PPAR-gamma by GW9662 could prevent the inhibition of 6-Shogaol on LPS-induced inflammatory mediator production. In conclusion, 6-Shogaol inhibits LPS-induced inflammation by activating PPAR-gamma.