Journal
ONCOTARGET
Volume 9, Issue 4, Pages 4338-4353Publisher
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.22774
Keywords
invasive micropapillary carcinoma; polarity reversal; beta 1 integrin; metastasis; breast
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Invasive micropapillary carcinoma (IMPC) of the breast is a highly aggressive breast cancer. Polarity reversal exemplified by cluster growth is hypothesized to contribute to the invasiveness and metastasis of IMPC. In this study, we demonstrate that levels of beta 1 integrin and Rac1 expression were greater in breast IMPC than in invasive breast carcinoma of no specific type and paraneoplastic benign breast tissue. We show that silencing beta 1 integrin expression using the beta 1 integrin inhibitor AIIB2 partially restored polarity in IMPC primary cell clusters and downregulated Rac1. Thus, overexpression of beta 1 integrin upregulates Rac1. Univariate analysis showed that overexpression of beta 1 integrin and Rac1 was associated with breast cancer cell polarity reversal, lymph node metastasis, and poor disease-free survival in IMPC patients. Multivariate analysis revealed that polarity reversal was an independent predictor of poor disease-free survival. These findings indicate that overexpression of beta 1 integrin and the resultant upregulation of Rac1 contribute to polarity reversal and metastasis of breast IMPC, and that beta 1 integrin and Rac1 could be potential prognostic biomarkers and targets for treatment of breast IMPC.
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