Journal
CHROMATOGRAPHIA
Volume 80, Issue 7, Pages 1025-1033Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10337-017-3317-z
Keywords
UPLC-ESI-MS/MS; Hedera helix; Ivy leaf extract; alpha-Hederin; Hederacoside C
Funding
- Global Leading Technology Program of the Office of Strategic R&D Planning (OSP) - Ministry of Knowledge Economy, Republic of Korea [10039321]
- Korea Evaluation Institute of Industrial Technology (KEIT) [10039321] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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We previously reported a method for simultaneously determining flavonoids and saponins from ivy leaf extract using HPLC-DAD. Due to the absence of a chromophore in two triterpene saponins, alpha-hederin and hederacoside C, the previous method was conducted at a non-specific wavelength of 210 nm. Thus, in this study, we focused on a rapid and sensitive analysis method for determining two important triterpene saponins from ivy leaf extract using UPLC-ESI-MS/MS. The developed method was validated in terms of linearity (R (2) > 0.9997), precision (RSD < 2.95%), and accuracy (98.91-101.92%), and showed increased sensitivity with improved limit of detection when compared to the previous data. In addition, the analytical conditions of UPLC-ESI-MS/MS provided better sensitivity with a shorter analysis time when compared to the HPLC-DAD method. Using this established method, the content of the six compounds was measured in medicinal syrups containing ivy leaf extract and the feasibility of this new method was demonstrated for the use in the quality control of ivy leaf extract products.
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