Journal
ONCOTARGET
Volume 8, Issue 16, Pages 26979-26991Publisher
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.15927
Keywords
PP2A-B55 alpha; oocyte maturation; cytokinesis; preimplantation development; reproduction
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Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2015R1D1A1A01057629]
- Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea [PJ011126]
- China Scholarship Council (CSC) [[2014]3026]
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Protein phosphatase 2A regulatory subunit B55 alpha (PP2A-B55 alpha) has been studied in mitosis. However, its functions in mammalian meiosis and early embryonic development remain unknown. Here, we report that PP2A-B55 alpha is critical for mouse oocyte meiosis and preimplantation embryo development. Knockdown of PP2A-B55 alpha in oocytes led to abnormal asymmetric division, disordered spindle dynamics, defects in chromosome congression, an increase in aneuploidy, and induction of the DNA damage response. Moreover, knockdown of PP2A-B55 alpha in fertilized mouse zygotes impaired development to the blastocyst stage. The impairment of embryonic development might have been due to induction of sustained DNA damage in embryos, which caused apoptosis and inhibited cell proliferation and outgrowth potential at the blastocyst stage. Overall, these results provide a novel insight into the role of PP2A-B55 alpha as a novel meiotic and embryonic competence factor at the onset of life.
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