Bioengineered mannan sulphate capped silver nanoparticles for accelerated and targeted wound healing: Physicochemical and biological investigations

In the present study, surface functionalized mannan sulphate silver nanoparticles (MS-AgNPs) were prepared and assessed for their wound healing potential. 20 nm sized, spherical MS-AgNPs were prepared by one pot synthesis approach wherein the sulphated polysaccharide mannan sulphate (MS) played dual role of reducing as well as capping agent. The crystalline MS-AgNPs exhibited surface plasmon resonance centered at 400 nm along with -32.40 mV zeta potential. These stable MS-AgNPs showed enhanced cytocompatibility, targeting potential and cellular uptake in murine macrophages, human skin fibroblasts and human keratinocytes as compared to citrate reduced silver nanoparticles (CAgNPs). In the in vivo excision and incision wound models, MS-AgNPs as hydrogel formulations indicated better efficacy than the conventional and marketed silver formulations. Thus, the synthesized MS-AgNPs depicted a promising potential for site-specific topical delivery in accelerated wound therapy. (C) 2017 Elsevier Masson SAS. All rights reserved.