Journal
EUROPEAN CELLS & MATERIALS
Volume 34, Issue -, Pages 55-69Publisher
AO RESEARCH INSTITUTE DAVOS-ARI
DOI: 10.22203/eCM.v034a04
Keywords
Integra (R); dermal substitute; skin; tissue engineering; wound healing; vascularisation; photoacoustic imaging; dorsal skinfold chamber
Categories
Funding
- Deutsche Forschungsgemeinschaft (DFG-German Research Foundation) [LA2682/7-1]
Ask authors/readers for more resources
Adipose tissue-derived microvascular fragments (ad-MVF) represent effective vascularisation units for the seeding of dermal substitutes. However, particularly in case of extensive skin defects, the required amounts of donor fat tissue for the harvesting of ad-MVF may not always be available. Therefore, we herein determined the lowest ad-MVF density needed to induce a sufficient vascularisation and incorporation of seeded implants. Collagen-glycosaminoglycan matrices (Integra (R); diameter: 4 mm) were seeded with 15,000 (HD), 10,000 (MD) and 5,000 (LD) ad-MVF and implanted into full-thickness skin defects within mouse dorsal skinfold chambers, to analyse their in vivo vascularisation and incorporation. Intravital fluorescence microscopy showed a comparable vascularisation of HD and MD ad-MVF-seeded Integra (R), which was significantly higher when compared to LD ad-MVF-seeded Integra (R). As assessed by photoacoustic imaging, this was associated with an increased oxygenation of the implants. Additional histological and immunohistochemical analyses revealed an enhanced cellular infiltration, collagen content, microvessel density and epithelialisation of HD and MD ad-MVF-seeded Integra (R), indicating a better incorporation compared to LD ad-MVF-seeded implants. These findings demonstrate that 80,000 ad-MVF/cm(2) is the least required density to guarantee an effective vascularisation of the dermal substitute.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available