Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 155, Issue -, Pages 150-158Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2017.04.012
Keywords
Reduced graphene oxide; Electrochemical deposition; Scaffold; Biocompatible; Bone regeneration
Funding
- National Natural Science Foundation of China [81601613]
- Tianjin Natural Science Foundation [16JCZDJC32800]
- Medical Scientific Research Foundation of Guangdong Province [A2016394]
- Science & Technology Support Program of Sichuan Province [2016FZ0085, 2015SZ0078]
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In this study, three-dimensional reduced graphene oxide (3D-rGO) porous films were fabricated using a two-step electrochemical method, including an electrochemical deposition process for the self-assembly of GO and an electrochemical bubbling-based transfer. The morphology, physical properties, and phase composition of the 3D-rGO films were characterized, and the cellular bioactivities were evaluated using pre-osteoblasts (MC3T3-E1 cells). The attachment, proliferation and differentiation of the MC3T3-E1 cells on the 3D-rGO films was analyzed by scanning electron microscopy (SEM), Cell Counting Kit-8 (CCK-8) assays and live/dead cell staining, and alkaline phosphatase (ALP) activity assays, respectively. The expression of osteogenic-related genes in MC3T3-E1 cells was evaluated by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the 3D-rGO films supported cell viability and proliferation, as well as significantly enhanced ALP activity and osteogenic-related genes (ALP, OPN, Runx2) expressions. Our findings indicate the promising potential of the 3D-rGO porous films for bone tissue engineering. (C) 2017 Elsevier B.V. All rights reserved.
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