Journal
ONCOTARGET
Volume 8, Issue 26, Pages 43114-43129Publisher
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.17810
Keywords
melanoma; prohibitin; mitochondria; cisplatin; tunicamycin
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Funding
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, FAPESP [1998/14247-6]
- National Institute for Science and Technology, Redox Processes in Biomedicine (Redoxoma) [CNPq 573530/2008-4]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [98/14247-6] Funding Source: FAPESP
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Melanoma is responsible for most deaths among skin cancers and conventional and palliative care chemotherapy are limited due to the development of chemoresistance. We used proteomic analysis to identify cellular responses that lead to chemoresistance of human melanoma cell lines to cisplatin. A systems approach to the proteomic data indicated the participation of specific cellular processes such as oxidative phosphorylation, mitochondrial organization and homeostasis, as well as the unfolded protein response (UPR) to be required for the survival of cells treated with cisplatin. Prohibitin (PHB) was among the proteins consistently accumulated, interacting with the functional clusters associated with resistance to cisplatin. We showed PHB accumulated at different levels in melanoma cell lines under stressing stimuli, such as (i) treatment with temozolomide (TMZ), dacarbazine (DTIC) and cisplatin; (ii) serum deprivation; (iii) tunicamycin, an UPR inducer. Prohibitin accumulated in the mitochondria of melanoma cells after cisplatin and tunicamycin treatment and its de novo accumulation led to chemoresistance melanoma cell lines. In contrast, PHB knockdown sensitized melanoma cells to cisplatin and tunicamycin treatment. We conclude that PHB participates in the survival of cells exposed to different stress stimuli, and can therefore serve as a target for the sensitization of melanoma cells to chemotherapy.
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