Journal
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
Volume 99, Issue 1, Pages 39-45Publisher
SPRINGER
DOI: 10.1007/s00128-017-2046-1
Keywords
Dydrogesterone; Zebrafish; Transcription; Biochemical alteration
Categories
Funding
- National Natural Science Foundation of China [41273119, U1401235]
- National Water Pollution Control Program [2014ZX07206-005]
- GIG CAS [IS-2348]
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Little information has so far been known on the effects of synthetic progestogen dydrogesterone (DDG) in organisms like fish. This study aimed to investigate the effects of DDG on the transcriptional and biochemical alterations in zebrafish eleuthero-embryos. Zebrafish eleuthero-embryos were analyzed for the transcriptional alterations by real-time quantitative PCR (RT-qPCR) and biochemical changes by attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FITR) after 144 h exposure to DDG. The results of qPCR analysis showed that DDG exposure significantly suppressed the transcriptions of target genes involved in hypothalamic-pituitary-thyroid (HPT) axis, while it induced the expression of target genes mRNA belonging to hypothalamic-pituitary-gonad (HPG) axis. In addition, ATR-FTIR spectroscopy analysis showed that the biochemical alterations of protein, nucleic acid and lipid were observed following DDG treatment. The finding from this study suggests that DDG exposure could have potential multiple effects in fish.
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