Journal
NATURE STRUCTURAL & MOLECULAR BIOLOGY
Volume 24, Issue 10, Pages 809-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nsmb.3465
Keywords
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Funding
- Deutsche Forschungsgemeinschaft [SFB860, SPP1935]
- European Research Council [693023]
- Volkswagen Foundation
- European Research Council (ERC) [693023] Funding Source: European Research Council (ERC)
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During transcription, RNA polymerase II (Pol II) associates with the conserved elongation factor DSIF. DSIF renders the elongation complex stable and functions during Pol II pausing and RNA processing. We combined cryo-EM and X-ray crystallography to determine the structure of the mammalian Pol II-DSIF elongation complex at a nominal resolution of 3.4 angstrom. Human DSIF has a modular structure with two domains forming a DNA clamp, two domains forming an RNA clamp, and one domain buttressing the RNA clamp. The clamps maintain the transcription bubble, position upstream DNA, and retain the RNA transcript in the exit tunnel. The mobile C-terminal region of DSIF is located near exiting RNA, where it can recruit factors for RNA processing. The structure provides insight into the roles of DSIF during mRNA synthesis.
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