Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 488, Issue 4, Pages 641-647Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2017.01.047
Keywords
ER stress; Membrane-tethered transcription factors; NAC-family transcription factor; Tunicamycin; Unfolded protein response
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Funding
- Basic Science Research Program through the National Research Foundation of Korea - Ministry of Education [2015R1D1A1A01061056]
- NG-BioGreen21 Program (SSAC), RDA, Korea [PJ011379]
- National Research Foundation of Korea [2015R1D1A1A01061056] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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We screened for endoplasmic reticulum (ER) stress-resistant mutants among 25 mutants of the Arabidopsis NTL (NAC with Transmembrane motif 1-Like) family. We identified a novel mutant, SALK_044777, showing strong resistance to ER stress. RT-PCR and genomic DNA sequence analyses identified the mutant as atntl7, which harbors a T-DNA insertion in the fourth exon of AtNTL7. Two other atntl7-mutant alleles, in which T-DNA was inserted in the second exon and third intron of AtNTL7, respectively, showed ER-stress sensitive phenotypes, suggesting that SALI_044777 is a gain-of-function mutant. Arabidopsis plants overexpressing AtNTL7 showed strong ER-stress resistance. Our findings suggest that AtNTL7 fragment is cleaved from the ER membrane under ER stress andtranslocates into the nucleus to induce downstream ER -stress responsive genes. (C) 2017 Elsevier Inc. All rights reserved.
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