Journal
EUROPEAN JOURNAL OF IMMUNOLOGY
Volume 45, Issue 4, Pages 1270-1273Publisher
WILEY
DOI: 10.1002/eji.201445247
Keywords
CD4(+) T cells; Cellular activation; Cytokines; Flow cytometry; PMA; ionomycin
Categories
Funding
- German Research Council [DFG SFB633, SFB 650, GRK1121]
- European Research Council [268987]
- European Research Council (ERC) [268987] Funding Source: European Research Council (ERC)
Ask authors/readers for more resources
Restimulation of memory-effector T helper lymphocytes with PMA/ionomycin is an established technique to determine their imprinting for the expression of cytokine genes, that is their functional potential. Here, we show that for the maximal reexpression of cytokine genes, the Ca2+ concentration of the medium has to be greater than 1.5 mM. For RPMI (Roswell Park Memorial Institute 1640), the most commonly used medium, the Ca2+ concentration has to be increased from the regular 0.42 to 1.5 mM.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available