Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 114, Issue 8, Pages 1791-1802Publisher
WILEY
DOI: 10.1002/bit.26270
Keywords
microfluidic cell sorting; CHO cells; recombinant protein expression; protein secretion; magnetic microparticles
Categories
Funding
- Kommission fur Technologie und Innovation [12114.1]
- Selexis SA
- Spinomix SA
- University of Lausanne
Ask authors/readers for more resources
We developed a method for the fast sorting and selection of mammalian cells expressing and secreting a protein at high levels. This procedure relies on cell capture using an automated microfluidic device handling antibody-coupled magnetic microparticles and on a timed release of the cells from the microparticles after capture. Using clinically compatible materials and procedures, we show that this approach is able to discriminate between cells that truly secrete high amounts of a protein from those that just display it at high levels on their surface without properly releasing it. When coupled to a cell colony imaging and picking device, this approach allowed the identification of CHO cell clones secreting a therapeutic protein at high levels that were not achievable without the cell sorting procedure. Biotechnol. Bioeng. 2017;114: 1791-1802. (c) 2017 Wiley Periodicals, Inc.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available