Journal
DEVELOPMENTAL BIOLOGY
Volume 426, Issue 2, Pages 255-260Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2016.05.015
Keywords
Xenopus tropicalis; Genomics; BAC library; Amphibian
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Funding
- Genopole (Centre d'Exploration et de Recherche Fonctionnelle Amphibiens Poissons)
- Association Francaise contre les Myopathies (Amphigenetools)
- Centre National de la Recherche Scientifique
- Universite d'Evry-Val d'Essonne
- Universite Paris-Sud
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Large insert genomic DNA libraries are useful resources for genomic studies. Although the genome of Xenopus tropicalis stands as the amphibian reference genome because it benefitted from large-scale sequencing studies, physical mapping resources such as BAC libraries are lagging behind. Here we present the construction and characterization of a BAC library that covers the whole X. tropicalis genome. We prepared this BAC library from the genomic DNA of X. tropicalis females of the Adiopodoume strain. We characterized BAC clones by screening for specific loci, by chromosomal localization using FISH and by systematic BAC end sequencing. The median insert size is about 110 kbp and the library coverage is around six genome equivalents. We obtained a total of 163,787 BAC end sequences with mate pairs for 77,711 BAC clones. We mapped all BAC end sequences to the reference X. tropicalis genome assembly to enable the identification of BAC clones covering specific loci. Overall, this BAC library resource complements the knowledge of the X. tropicalis genome and should further promote its use as a reference genome for developmental biology studies and amphibian comparative genomics. (C) 2017 Elsevier Inc. All rights reserved.
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