Journal
DEVELOPMENT
Volume 144, Issue 13, Pages 2352-2363Publisher
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/dev.151613
Keywords
Embryo; Chicken; Aorta; Hematopoietic stem cells; Hematopoietic clusters; Chorio-allantoic membrane transplantation
Categories
Funding
- European Research Council grant (ERC) [220-H75001EU/HSCOrigin-309361]
- TOP-subsidy from the Nederlandse Organisatie voor Wetenschappelijk Onderzoek/ZonMw [912.15.017]
- Universitair Medisch Centrum Utrecht 'Regenerative Medicine & Stem Cells' priority research program
Ask authors/readers for more resources
Hematopoietic stem cells (HSCs), which are responsible for blood cell production, are generated during embryonic development. Human and chicken embryos share features that position the chicken as a reliable and accessible alternative model to study developmental hematopoiesis. However, the existence of HSCs has never been formally proven in chicken embryos. Here, we have established a complete cartography and quantification of hematopoietic cells in the aorta during development. We demonstrate the existence of bona fide HSCs, originating from the chicken embryo aorta (and not the yolk sac, allantois or head), through an in vivo transplantation assay. Embryos transplanted in ovo with GFP embryonic tissues on the chorio-allantoic membrane provided multilineage reconstitution in adulthood. Historically, most breakthrough discoveries in the field of developmental hematopoiesis were first made in birds and later extended to mammals. Our study sheds new light on the avian model as a valuable system to study HSC production and regulation in vivo.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available