Journal
EUROPEAN JOURNAL OF HISTOCHEMISTRY
Volume 59, Issue 1, Pages 61-65Publisher
PAGEPRESS PUBL
DOI: 10.4081/ejh.2015.2492
Keywords
Nanoparticles; endosomal escape; cell nucleus; DAB photo-oxidation
Categories
Funding
- Fondazione Cariverona, project Verona Nanomedicine Initiative
- Doctoral Program of the University of Pavia
- Doctoral Program of the University of Verona
- project Verona Nanomedicine Initiative
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Chitosan-based nanoparticles (chiNPs) are considered to be potentially good carriers for the sustained intracellular delivery of specific molecules. However, scarce attention has been paid to the long-lasting permanence of these NPs in the intracellular milieu, as well as to their intracellular fate (i.e., distribution, interaction with cell organelles, and degradation) in the long term. In the present study, the presence and subcellular location of FITC-labelled chiNPs were monitored in HeLa cells up to 14 days post-administration using multicolor-fluorescence confocal microscopy and diaminobenzidine photo-oxidation at transmission electron microscopy. The main result of the present study is the demonstration that internalized chiNPs persist inside the cell up to two weeks, occurring in both the cytoplasm and nucleus; accordingly, chiNPs are able to pass from mother to daughter cells through several mitotic cycles. The cells did not show increased mortality or structural damage up to 14 days after chiNP exposure.
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