Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 123, Issue -, Pages 86-94Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bej.2017.03.015
Keywords
Biosensors; Immobilized cells; Dissolved oxygen; Mass transfer; Microbial electrode; Carboxyl graphene
Funding
- National Basic Research Program of China (973 Program) [2015CB352100]
- National Natural Science Foundation of China [61401433]
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Biochemical oxygen demand (BOD) is an international regulatory index for evaluating organic water pollution, which requires to be rapidly detected. In this paper, a microbial electrode for rapid BOD sensing has been developed. Carboxyl graphene (GN-COOH) and Au nanoparticles (AuNPs) have been co-electrodeposited on the electrode and microorganisms (Bacillus subtilis) have been immobilized onto the modified electrode through covalent bonding. The modification process has been investigated by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscope (SEM), fluorescence microscope and Fourier transform infrared spectroscopy (FT-IR). The AuNPs with an average particle size of 30 nm are dispersed in the GN-COOH film. The co-electrodeposited film not only facilitates the electron transfer between electrode and reagents, but also creates a biocompatible microenvironment and a rough surface with more sites for cell immobilization. Compared with the traditional BOD electrode including immobilized microbial film and oxygen permselective membrane, the B. subtilis/rGN-COOH-AuNPs/Au electrode simplifies the structure for more effective mass transfer. After modification parameters' optimization, the response time for detection has been shortened to 3 min. The proposed BOD electrode shows a linear range from 2 to 15 mg/L, indicating its potential in BOD rapid detection. (C) 2017 Published by Elsevier B.V.
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