Journal
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
Volume 1862, Issue 8, Pages 777-781Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbalip.2017.03.008
Keywords
Mass spectrometer; Quantification; Eicosanoids; Cross-platform
Funding
- Ono Pharmaceutical Co., Ltd.
- Shimadzu, Corp.
- Ministry of Education, Culture, Sports, Science and Technology, Japan [24229003, 15K08296]
- Grants-in-Aid for Scientific Research [15K08296] Funding Source: KAKEN
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Comprehensive quantitative analysis of lipid mediators using liquid chromatography-tandem mass spectrometry is an effective strategy in the elucidation of disease mechanisms; but technically, it has been and is still a great challenge to achieve reliable datasets that cover variety of lipid metabolites contained at trace levels in complex biological matrices. In this opinion article, we introduce our experiences in developing lipid mediator profiling systems, and deliver some comments on limitations of current methodology.
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