Journal
ADVANCED FUNCTIONAL MATERIALS
Volume 27, Issue 26, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201701473
Keywords
adhesin; alternating magnetic field; E. coli; glycoconjugate; magnetic nanoparticles
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Funding
- NIFA/USDA [SC-1700430]
- Consortium for Functional Glycomics (CFG grant) [3223]
- National Science Foundation [CMMI-1057633, CMMI-1130819]
- Clemson University Calhoun Honors College
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New technologies that do not rely on antibiotics are urgently needed to treat bacterial infections caused by multidrug-resistant bacteria. Herein, the feasibility of using alternating magnetic field (AMF) to selectively kill enterotoxigenic Escherichia coli strain K99 (EC K99) in the presence of multianchored glycoconjugate-functionalized magnetic nanoparticles is explored. Poly(ethylene oxide)-poly(acrylic acid)-dopamine functionalized magnetic nanoparticles (PEO-MNPs) are synthesized and functionalized with bacteria-specific glycoconjugate Neu5Ac(alpha 2-3)-Gal-(beta 1-4) Glc beta-sp (GM3-MNPs) for specific adherence to EC K99. When such mixtures are exposed to an alternate magnetic field (31 kA m(-1), 207 KHz), an approximate to 3-log reduction in colony forming units of EC K99 is achieved in 120 min. Moreover, in a mixed-bacterial culture environment, targeted killing of EC K99 is achieved with minimal damage to nontargeted bacterium. Electron microscopy images along with live/dead staining assays demonstrate visible membrane damage of EC K99 cells in the presence of GM3-MNPs and AMF. Additionally, intracellular adenosine triphosphate (ATP) levels of EC K99 are significantly diminished in the presence of GM3-MNPs and AMF. These results suggest that specific glycoconjugate-functionalized magnetic nanoparticles when mediated by AMF can be potentially used as a novel nonantibiotic treatment platform to inactivate/kill targeted bacterial pathogens, with minimal impact on normal microflora and the affected body region/tissue.
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