Journal
SOIL & ENVIRONMENT
Volume 36, Issue 1, Pages 35-44Publisher
UNIV AGRICULTURE, INST SOIL & ENVIRONMENTAL SCIENCES
DOI: 10.25252/SE/17/20992
Keywords
Biosurfactant; TLC; FT-IR; 16s rRNA sequencing; heavy metals
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Funding
- University of the Punjab, Lahore, Pakistan
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Biosurfactants are microbial amphiphilic compounds which can reduce surface tension between aqueous and hydrocarbon mixtures. Bacterial strains isolated from petroleum contaminated soil of various motor workshops were characterized morphologically and biochemically. Biosurfactant producing ability of the strains was determined and their emulsification activity was screened against different oils. All the selected bacterial strains showed enhanced biosurfactants production with yeast extract as nitrogen source and glucose as carbon source at optimized conditions. These strains also exhibited multiple metal and antibiotics resistance. Isolated biosurfactants of three most promising strains SF-1, SF-4 and SM-1 were extracted by solvent extraction and subjected to TLC technique. The technique indicates the glycolipid nature of the compounds and presence of rhamnose sugar, which was further confirmed by FT-IR analysis. 16srRNA analysis revealed that SF-1 and SM-1 had close resemblance with Pseudomonas sp. while SF-4 showed homology with Enterobacter sp. Isolation and screening of biosurfactant producing strains from petroleum polluted places proved to be a quick and effective means to find bacterial strains with possible industrial uses.
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