Soluble soybean polysaccharides enhance the protective effects of genistein against hepatic injury in high L-carnitine-fed mice

This study was to develop a novel strategy for the simultaneous consumption of soluble soybean polysaccharides (SSPS) to enhance the absorption of genistein and its protective effects against high L-carnitine-induced hepatic injury in mice. UPLC-qTOP/MS measurements showed that SSPS observably increased the urinary concentration of genistein and its metabolites in mice. The mice fed with 3% L-carnitine water for 12 weeks experienced a disturbance of the hepatic lipid metabolism, oxidative stress and inflammation, which was evidenced by abnormal TC, LDL, RAHFR and MDA levels, unusual AST, ALT, ALP, SOD and GSP-Px activities, and increased IF-1, IF-6 and TNF-alpha expressions. Interestingly, the co-supplementation of SSPS and genistein was capable of regulating these imbalances more effectively than the administration of SSPS or genistein alone, which was also confirmed by histological observations of the mouse liver. These findings suggest that the co-ingestion of SSPS and genistein is a feasible strategy for improving liver protection in mice.