Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 156, Issue -, Pages 375-381Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2017.05.036
Keywords
Structure and stability; Magnetoferritin solution; pH-dependent structure; Small-angle X-ray scattering; Dynamic light scattering
Funding
- Slovak Scientific Grant Agency VEGA [2/0016/17]
- Slovak Research and Development Agency [APVV-015-0453]
- Ministry of Education Agency for European Structural funds [26220120033, 26210120012, 26220220186]
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The structural stability of magnetoferritin, a synthetic analogue of ferritin, at various pH levels is assessed here. The structural and electrical properties of the complexes were determined by small-angle X-ray scattering (SAXS), dynamic light scattering (DLS) and zeta potential measurements. At pH 3-6 a reduction of electrostatic repulsion on the suspended colloids resulted in aggregation and sedimentation of magnetoferritin. At neutral to slightly alkaline conditions (pH 7-9) the magnetoferritin structure was stable for lower iron loadings. Higher solution pH 10-12 induced destabilization of the protein structure and dissociation of subunits. Increasing the loading factor in the MFer complex leads to decrease of the stability versus pH changes. (C) 2017 Elsevier B.V. All rights reserved.
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