Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 156, Issue -, Pages 213-220Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2017.05.011
Keywords
Hierarchical porous structure; Bioglass films; MC3T3-E1; rhBMP-2 loading; Osteogenic differentiation
Funding
- National Natural Science Foundation of China [51272228, 81271955, 51472216, 51372217]
- National Basic Research Program of China (973 Program) [2012CB933600]
- Key Science Technology Innovation Team of Zhejiang Province [2013TD02]
- Natural Science Foundation of Zhejiang [LY15E020004]
- Fundamental Research Funds for the Central Universities [2013QNA4010, 2014XZZX005]
Ask authors/readers for more resources
Hierarchical porous bioglass films on the tantalum were designed to enhance osteointegration of metallic implants. The films were prepared by a sol-gel method using P123 as the mesopore template and polystyrene microsphere as the nanopore template. The films with 5.4 nm mesopores and 100nm nanopores (MBG-100) elicited an obviously elongated morphology of the cultured MC3T3-E1 cells, as a result, a higher alkaline phosphatase level was expressed. It is suggested that the nanopores play an important role in regulating cellular behavior by initial protein adsorption through nanopore curvatures. The mesopores were proven very effective for loading rhBMP-2, and the rhBMP-2 loaded on MBG-100 films showed a better function of enhancing osteogenic differentiation, which is attributed to that the nanopore structure could expedite rhBMP-2 release and provide a microenvironment for intensifying the interaction of rhBMP-2 with the cells. Hence, the cell osteogenic differentiation can be enhanced by hierarchical porous bioglass films through both the porous structure and rhBMP-2 induction. (C) 2017 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available