Journal
BIORESOURCE TECHNOLOGY
Volume 241, Issue -, Pages 692-700Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.05.208
Keywords
Cotton stalk; Steam explosion; Glucan conversion; Xylo-oligomers; Enzymatic saccharification
Funding
- Department of Biotechnology (DBT), India [BT/PB/08/03/2007]
- IOC-DBT Centre for Advanced Bioenergy Research
- Indian Oil Corporation Limited (RD Centre)
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Dilute acid (DA) pretreatment at pilot level failed for cotton stalk (CS) due to the technical issues posed by its inherent nature. Reasonable glucan conversion has been reported via two-stage pretreatment but adds on to the process cost. Proposed herewith is a single-stage steam explosion (SE) process preceded by water extraction resulting in high sugar recovery from CS. Raising the extraction temperature to 80 degrees C increased the glucan conversion from 37.9 to 52.4%. Further improvement up to 68.4% was achieved when DA was incorporated during the room temperature extraction. LC-MS revealed the formation of xylo-oligomers limiting the glucan conversion in proportion to the length of xylo-oligomers. Varying extraction conditions induced structural alterations in biomass after SE evident by compositional analysis, Infrared Spectroscopy, X-Ray Diffraction and Scanning Electron Microscopy. Overall glucose recovery, i.e. 75.8-76.7% with and without DA extraction respectively was achieved. (C) 2017 Elsevier Ltd. All rights reserved.
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