Journal
BIORESOURCE TECHNOLOGY
Volume 241, Issue -, Pages 252-261Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2017.05.118
Keywords
Fed batch; Recycle; Soy flour; Fermentation feedstock; Soy protein; Bio-refinery
Funding
- United Soybean Board [1475, 2475, 5256]
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Despite having high protein and carbohydrate, soybean flour utilization is limited to partial replacement of animal feed to date. Enzymatic process can be exploited to increase its value by enriching protein content and separating carbohydrate for utilization as fermentation feedstock. Enzyme hydrolysis with fed-batch and recycle designs were evaluated here for achieving this goal with high productivities. Fed-batch process improved carbohydrate conversion, particularly at high substrate loadings of 250-375 g/L. In recycle process, hydrolysate retained a significant portion of the limiting enzyme alpha-galactosidase to accelerate carbohydrate monomerization rate. At single-pass retention time of 6 h and recycle rate of 62.5%, reducing sugar concentration reached up to 120 g/L using 4 ml/g enzyme. When compared with batch and fed-batch processes, the recycle process increased the volumetric productivity of reducing sugar by 36% (vs. fed-batch) to 57% (vs. batch) and that of protein product by 280% (vs. fed-batch) to 300% (vs. batch). (C) 2017 Elsevier Ltd. All rights reserved.
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