Journal
BREASTFEEDING MEDICINE
Volume 12, Issue 6, Pages 365-372Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/bfm.2016.0097
Keywords
smokers' breast milk; cholesterol; apolipoprotein A-I; antioxidant; zebrafish embryo
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Funding
- Mid-Carrier Researcher Program [2014-11049455]
- Medical Research Center Program through the National Research Foundation (NRF) - Ministry of Science, ICT, and Future Planning of Korea [2015R1A5A2009124]
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Background: To determine the quality of breast milk (BM), we compared the functions of BM from ex-smokers and nonsmokers. Subjects and Methods: We analyzed the contents of lipids, glucose, and protein in BM from ex-smokers (10 cigarettes/day for 133 years) as well as infant formula. Results: Nonsmokers' BM showed 2.4- and 1.4-fold higher cholesterol and protein contents, respectively, than BM from smokers. Infant formula contained almost no cholesterol, but did show remarkably higher glucose and triglyceride levels than BM. Microinjection of BM (50nL) from nonsmokers and smokers into zebrafish embryos resulted in 59% and 44% survival, respectively, whereas formula injection resulted in 31% survival. The higher cholesterol and protein contents of BM were directly correlated with higher embryo survivability, suggesting that cholesterol content is directly and critically associated with growth of neonate infants. Smokers' BM contained smaller-sized apolipoproteinA-I (apoA-I) (24.4 +/- 0.2kDa) than BM from nonsmokers (26.7 +/- 0.4kDa), suggesting that putative modification and cleavage occurred in apoA-I. BM containing higher molecular weight apoA-I resulted in higher embryo survivability. Conclusions: Smoking before pregnancy can affect the composition and quality of BM, resulting in almost complete loss of cholesterol and protein, especially lactoferrin, lactalbumin, and apoA-I, accompanied by proteolytic degradation. These impairment effects of BM are associated with elevation of oxidative stress and lower embryo survivability.
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