Journal
EUROPEAN JOURNAL OF IMMUNOLOGY
Volume 47, Issue 7, Pages 1136-1141Publisher
WILEY
DOI: 10.1002/eji.201747034
Keywords
Bcl6; Blimp1; Follicular helper T cells; Germinal center; PD-1; T-bet; T follicular regulatory cells
Categories
Funding
- NIAID [1R21AI099825, R03 AI110987, R03AI119623]
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The transcription factors Bcl6 and Blimp1 have opposing roles in the development of the follicular helper T (T-FH) cells: Bcl6 promotes and Blimp1 inhibits T-FH-cell differentiation. Similarly, Bcl6 activates, while Blimp1 represses, expression of the T-FH-cell marker PD-1. However, Bcl6 and Blimp1 repress each other's expression, complicating the interpretation of the regulatory network. Here we sought to clarify the extent to which Bcl6 and Blimp1 independently control T-FH-cell differentiation by generating mice with T-cell specific deletion of both Bcl6 and Blimp1 (double conditional KO [dcKO] mice). Our data indicate that Blimp1 does not control T-FH-cell differentiation independently of Bcl6. However, a population of T follicular regulatory (T-FR) cells developed independently of Bcl6 in dcKO mice. We have also analyzed regulation of IL-10 and PD-1, two genes controlled by both Bcl6 and Blimp1, and observed that Bcl6 regulates both genes independently of Blimp1. We found that Bcl6 positively regulates PD-1 expression by inhibiting the ability of T-bet/Tbx21 to repress Pdcd1 transcription. Our data provide a novel mechanism for positive control of gene expression by Bcl6, and illuminate how Bcl6 and Blimp1 control T-FH-cell differentiation.
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