Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 409, Issue 20, Pages 4849-4865Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-017-0428-1
Keywords
Pseudotargeted metabolomics; Citrus TCMs; Metabolites identification; Drug metabolism; QC-markers
Funding
- National Science Foundation of China [81573569, 81470177]
- Ministry of Finance of PRC
- Ministry of Education of PRC
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Traditional Chinese medicine (TCM) exerts its therapeutic effect in a holistic fashion with the synergistic function of multiple characteristic constituents. The holism philosophy of TCM is coincident with global and systematic theories of metabolomics. The proposed pseudotargeted metabolomics methodologies were employed for the establishment of reliable quality control markers for use in the screening strategy of TCMs. Pseudotargeted metabolomics integrates the advantages of both targeted and untargeted methods. In the present study, targeted metabolomics equipped with the gold standard RRLC-QqQ-MS method was employed for in vivo quantitative plasma pharmacochemistry study of characteristic prototypic constituents. Meanwhile, untargeted metabolomics using UHPLC-QE Orbitrap HRMS with better specificity and selectivity was employed for identification of untargeted metabolites in the complex plasma matrix. In all, 32 prototypic metabolites were quantitatively determined, and 66 biotransformed metabolites were convincingly identified after being orally administered with standard extracts of four labeled Citrus TCMs. The global absorption and metabolism process of complex TCMs was depicted in a systematic manner.
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