Journal
DATA IN BRIEF
Volume 13, Issue -, Pages 320-325Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.dib.2017.05.049
Keywords
MKK3; Cigarette smoke; Inflammation; Proteomics; iTRAQ (R)
Categories
Funding
- NCRR NIH HHS [S10 RR031795] Funding Source: Medline
- NHLBI NIH HHS [R01 HL090660, R01 HL071595] Funding Source: Medline
- NIA NIH HHS [P30 AG021342, R03 AG042358] Funding Source: Medline
Ask authors/readers for more resources
This data article reports changes in the phosphoproteome and total proteome of cigarette smoke extract (CSE) exposed WT and MAP Kinase Kinase 3 knock out (MKK3(-/-)) bone marrow derived macrophages (BMDM). The dataset generated is helpful for understanding the mechanism of CSE induced inflammation and the role of MAP kinase signaling pathway. The cellular proteins were labeled with isobaric tags for relative and absolute quantitation (iTRAQ (R)) reagents and analyzed by LC-MS/MS. The standard workflow module for iTRAQ quantification within the Proteome Discoverer was utilized for the data analysis. Ingenuity Pathway Analysis (IPA) software and Reactome was used to identify enriched canonical pathways and molecular networks (Mannam et al., 2016) [1]. All the associated mass spectrometry data has been deposited in the Yale Protein Expression Database (YPED) with the web-link to the data: http://yped.mned.yale.edu/repository/View SeriesMenu.do;jsessionid = 6A5CG075213D8B529FAE8C3FTFE292171 D?series_id = 5044&series_name=MMK3 + Deletion + in MEFs (C) 2017 The Authors. Published by Elsevier Inc.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available