Journal
BIOMEDICAL OPTICS EXPRESS
Volume 8, Issue 9, Pages 4108-4121Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.8.004108
Keywords
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Funding
- National Natural Science Foundation of China (NSFC) [61335011, 61405036, 61575043]
- Strait United Funding Project [U1605253]
- Major Projects of International Cooperation [61210016]
- National Key Basic Research Program of China [2015CB352006]
- Natural Science Foundation of Fujian Province of China [2017J01499, 2017J01843, 2017J01844, 2015J01242]
- Project of the Educational Office of Fujian Province [JA15233]
- Program for New Century Excellent Talents in University of Education Bureau of Fujian Province of China
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In this study, an electroporation-based surface-enhanced Raman scattering (SERS) technique was employed to differentiate the human myeloid leukemia cells from the normal human bone marrow mononuclear cells with the aim to develop a fast and label-free method for leukemia cell screening. The Ag nanoparticles were delivered into living cells by electroporation, and then high quality SERS spectra were successfully obtained from 60 acute promyelocytic leukemia cells (HL60 cell line), 60 chronic myelogenous leukemia cells (K562 cell line) and 60 normal human bone marrow mononuclear cells (BMC). Principal component analysis (PCA) combined with linear discriminant analysis (LDA) differentiated the leukemia cell SERS spectra (HL60 plus K562) from normal cell SERS spectra (BMC) with high sensitivity (98.3%) and specificity (98.3%). Furthermore, partial least squares (PLS) approach was employed to develop a diagnostic model. The model successfully predicted the unidentified subjects with a diagnostic accuracy of 96.7%. This exploratory work demonstrates that the electroporation-based SERS technique combined with PCA-LDA and PLS diagnostic algorithms possesses great promise for cancer cell screening. (C) 2017 Optical Society of America
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