Journal
BIOMEDICAL OPTICS EXPRESS
Volume 8, Issue 3, Pages 1455-1465Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.8.001455
Keywords
-
Funding
- National Institutes of Health [R01 CA138653, R03 CA191860]
- NPRP grant from the Qatar National Research Fund [8-1606-3-322]
Ask authors/readers for more resources
In this paper, we demonstrate the ability of structured illumination microscopy to enhance the ability of fluorescence lifetime imaging to resolve fluorescence lifetimes in relatively thick samples that possess distinct but spectrally overlapping fluorescent layers. Structured illumination fluorescent lifetime imaging microscopy (SI-FLIM) is shown to be able to accurately reconstruct lifetime values in homogenous fluorophore samples (POPOP, NADH, and FAD) as well as accurately measure fluorescent lifetime in two layer models that are layered with NADH/FAD over POPOP, where NADH/FAD and POPOP have spectral overlap. Finally, the ability of SI-FLIM was demonstrated in a hamster cheek pouch ex vivo to show that more accurate lifetimes could be measured for each layer of interest in the oral mucosa (epithelium and submucosa). (C) 2017 Optical Society of America
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available