Journal
BIOMEDICAL OPTICS EXPRESS
Volume 8, Issue 6, Pages 2796-2806Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.8.002796
Keywords
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Funding
- Research Foundation for Opto-Science and Technology
- JST/PRESTO [JPMJPR14F2]
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Temporal focusing (TF) microscopy is a wide-field two-photon excitation fluorescence (2PEF) microscopy technique, the optical sectioning capability of which is lower than that of point-scanning 2PEF microscopy. Here we demonstrate TF microscopy using three-photon excitation fluorescence (3PEF), which enhances the optical sectioning capability. As an excitation light source for the 3PEF, we developed an Yb-fiber chirped pulse amplifier, which produces 92-fs 9.0-mu J 1060-nm pulses at a repetition rate of 200 kHz. The optical sectioning capability was improved by a factor of 1.3 compared with that of 2PEF-TF microscopy. We also demonstrate dual-color imaging with both 2PEF and 3PEF. (C) 2017 Optical Society of America
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